Soy in Asia is just about everywhere and in most everything. It's as solid a staple as starches like rice or wheat, and while not as popular a protein choice as pork, it's pretty prevalent.
What I wanted to expand upon is geared more toward Shoyu-醤油(JPN)/Jiangyou-醬油(CHN)/Ganjang-간장(KOR)/Soy sauce, so I have to start at the beginning, and detail some of how it's made. The key ingredient is the koji mold, or aspergillus oryzae, which occurs naturally on rice, and has been utilized for thousands of years in various applications.
Japanese-style, the process of making soy sauce (and often miso too, because the technique is similar) is fairly simple. Steam your soybeans, combine with some (usually) toasted wheat, add some koji spores, and set it in a high-humidity environment for a few days until the koji multiplies and it all gets a little fuzzy.
That's the base. It gets mixed with salt and water to make a mash (諸味/moromi, a term also used for the mash used in brewing sake), then it hangs around for as many months or years as you've got. To keep air contact more even, it gets regularly stirred, too.
Once the brewer's intended fermentation period is up, the moromi will be (in cheesecloth, muslin, or other porous thing) packed, racked, stacked, and pressed. What comes out of the mash under that pressure is the baseline raw soy sauce, that's then gently cooked to stop the fermentation process, which presumably also helps stabilize the flavor to some degree.
That's soy sauce in brief. Now for some common variants.
In Japan, you're likely to see these:
- Tamari, made the same way but without the wheat- thus, gluten free
- Usukuchi / 'Light' soy sauce
- Koikuchi / 'Dark' soy sauce (The common Western supermarket stuff)
- Shiro / 'White' soy sauce, made with more wheat for a lighter color
- Light soy sauce (Comparable to the Japanese Koikuchi for utility, if not exact flavor)
- Dark soy sauce (Often sweetened with something, this is Very Intense, and it stains hard)
Your perceptions of flavour differences between tamari and wheat-containing o-shoyu ? -- How much antigenically intact gluten REALLY makes it through prolonged fermentation ?
ReplyDeleteJ Food Prot
Delete. 2017 May 1;80(5):799-808. doi: 10.4315/0362-028X.JFP-16-483.
Detection of Gluten during the Fermentation Process To Produce Soy Sauce
Wanying Cao 1 , Damien Watson 2 , Mikio Bakke 3 , Rakhi Panda 1 , Binaifer Bedford 2 , Parnavi S Kande 1 , Lauren S Jackson 4 , Eric A E Garber 5
PMID: 28371594 DOI: 10.4315/0362-028X.JFP-16-483
Abstract
Advances have been made to provide people with celiac disease (CD) access to a diverse diet through an increase in the availability of gluten-free food products and regulations designed to increase label reliability. Despite advances in our knowledge regarding CD and analytical methods to detect gluten, little is known about the effects of fermentation on gluten detection. The enzyme-linked immunosorbent assay (ELISA) and lateral flow devices routinely used by analytical laboratories and regulatory agencies to test for the presence of gluten in food were examined for their ability to detect gluten during the fermentation processes leading to the production of soy sauce, as well as in finished products. Similar results were observed irrespective of whether the soy sauce was produced using pilot-plant facilities or according to a homemade protocol. In both cases, gluten was not detected after moromi (brine-based) fermentation, which is the second stage of fermentation. The inability to detect gluten after moromi fermentation was irrespective of whether the assay used a sandwich configuration that required two epitopes or a competitive configuration that required only one epitope. Consistent with these results was the observation that ELISA, lateral flow devices, and Western immunoblot analyses were unable to detect gluten in commercial soy sauce, teriyaki sauce, and Worcestershire sauce. Although reports are lacking on problems associated with the consumption of fermented soy-containing sauces by consumers with CD, additional research is needed to determine whether all immunopathogenic elements in gluten are hydrolyzed during soy sauce production.
Keywords: Detection; Fermented; Gluten; Soy sauce.
* * *
Food Chem
. 2018 Jul 15;254:302-308. doi: 10.1016/j.foodchem.2018.02.023. Epub 2018 Feb 7.
Using LC-MS to examine the fermented food products vinegar and soy sauce for the presence of gluten
Haili Li 1 , Keren Byrne 2 , Renata Galiamov 2 , Omar Mendoza-Porras 2 , Utpal Bose 2 , Crispin A Howitt 3 , Michelle L Colgrave 4
PMID: 29548457 DOI: 10.1016/j.foodchem.2018.02.023
Abstract
A strict, lifelong gluten-free (GF) diet is currently the only treatment for coeliac disease (CD). Vinegar and soy sauce are fermented condiments that often include wheat and/or barley. During fermentation cereal proteins are partially degraded by enzymes to yield peptide fragments and amino acids. Whether these fermented products contain intact or degraded gluten proteins and if they are safe for people with CD remains in question. LC-MS offers the benefit of being able to detect hydrolysed gluten that might be present in commercial vinegar and soy sauce products. LC-MS revealed the presence of gluten in malt vinegar, wherein the identified peptides derived from B-, D- and γ-hordein from barley, as well as γ-gliadin, and HMW- and LMW-glutenins from wheat that are known to contain immunopathogenic epitopes. No gluten was detected in the soy sauces examined despite wheat being a labelled ingredient indicating extensive hydrolysis of gluten during soy sauce production.
Keywords: Fermented; Gluten; Hydrolysed; LC-MS; Mass spectrometry; Proteomics; Soy sauce; Vinegar.